Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4512125 | Industrial Crops and Products | 2016 | 6 Pages |
•Pasteurization led to an increase on total phenols and flavonoids content in jambolan pulp.•Anthocyanins content was reduced when compared with jambolan pulp without thermal treatment.•Jambolan pulp without pasteurization showed inhibition for melanoma lineage and resistant ovary.•Pasteurized jambolan pulp revealed cytostatic activities for kidney lineages and ovary.
Here we analyze jambolan pulp phenolic compounds in order to establish a correlation with antioxidant and in vitro anti-proliferative effects, both before and after pasteurization. Total levels of phenolic compounds, flavonoids and anthocyanins were quantified using UV–vis techniques. Major phenolic compounds were identified by standard compound co-injection in HPLC-DAD/UV–vis. Antioxidant activity was measured by radical scavenging ability, as determined by DPPH assay. In vitro anti-proliferative activity was determined against nine human tumour cell lines using the methodology described by the Developmental Therapeutics Program at NCI/NIH. Pasteurization led to an increase in the levels of total soluble solids (6.7%), phenolic compounds (7.2%) and flavonoids (16.4%). Anthocyanin content was largely preserved (91%) when compared with pulp without treatment. S. cumini preserved 56% of its original antioxidant activity after pasteurization, while thermal treatment revealed cytostatic activity in kidney (786-0) and ovary (OVCAR-3) lineages. Therefore, pasteurization can be applied successfully to S. cumini pulp.