| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4512138 | Industrial Crops and Products | 2016 | 13 Pages |
•A good range of variation was observed for different agro-morphological traits.•First four principal components explained 99.30% of the total variations in the data.•SSR markers found to be more polymorphic (PIC = 0.32, Polymorphism = 96.87%) than ISSRs in roselle accessions.•Different diversity parameters, structure and cluster analysis employed in the present study most convincingly demonstrated higher genetic diversity of fibre type roselle accessions than calyx type.•Morphological qualitative and molecular markers complemented each other and imparted greater resolution to understand genetic diversity of roselle plant genetic resources.
Hibiscus sabdariffa L. is a bast fibre crop bearing edible calyx. In the present study we characterized geographically diverse accessions (124) of H. sabdariffa var. altissima (HSA) and H. sabdariffa var. sabdariffa (HSS) using 23 morpho-metric (10 qualitative, 13 quantitative) traits and 19 microsatellite based markers (11 ISSRs; 8 SSRs). Wide spectrum diversity for agro-morphological traits recorded. Principal component analysis using 13 quantitative traits explained 99.30% of the total variation in the data by first four axes. A total of 91 polymorphic bands were yielded by microsatellite based markers (60 by ISSRs and 31 by SSRs) from them SSRs revealed more polymorphism (PIC = 0.32, polymorphism = 96.87%). The ISSR marker namely U818 exhibited highest primer index (ISPI = 2.275) whereas, SSR marker HA-8 reported highest PIC value (0.495). However, the highest genetic diversity at species level exhibited by ISSR markers (Shannon’s index = 0.519, heterozygosity = 0.353). Different diversity parameters, structure and cluster analysis employed in the present study demonstrated more genetic diversity of fibre type roselle accessions (HSA) than calyx type (HSS). Moreover, employed agro-morphological traits and molecular markers complemented each other and imparted greater resolution to understand genetic diversity of roselle plant genetic resources in toto.
