Article ID Journal Published Year Pages File Type
4513533 Industrial Crops and Products 2014 7 Pages PDF
Abstract

•The hydroalcoholic extraction of phenolics from black currant leaves was investigated.•Phenolics were more effectively extracted with 40% ethanol as compared with 80 °C water or 80% ethanol.•The highest contents of phenolics were recorded in mid-June for all cultivars.•Three flavonoids and seven phenolic acids were quantified by HPLC in black currant leaves.•In mid-june the leaves registered maximum contents of Ca, Mg, Fe, Mg, Al, Cr and B.

The efficiencies of 80 °C water, 40% ethanol and 80% ethanol in the extraction of phenolics from black currant leaves, as well as the antioxidant capacity of the obtained extracts, were investigated. Aqueous ethanol (40%) was the most effective in the extraction of phenolics followed by 80 °C water while the antioxidant capacity of the investigated extracts correlated with their phenolic content. Seven phenolic acids (gallic, chlorogenic, caffeic, p-coumaric, ferulic, sinapic and salicylic) and three flavonoids (rutin, myricetin and quercetin) were identified and quantified using HPLC with PDA detector. Also, total phenolics, minerals and trace elements content and antioxidant capacity were determined in black currant leaves of six different cultivars, over five harvesting dates (from June to August). Significant differences were observed among cultivars and sampling dates but the patterns of variation during the harvesting period were similar for all cultivars. The highest total phenolic contents and antioxidant capacities among samplings were recorded in mid-June for all the investigated cultivars, followed by a considerable decrease until early August. The black currant leaves registered maximum contents of Ca, Mg, Fe, Mg, Al, Cr and B in mid-June while the highest content of K was reached on June 1st. The results suggested that black currant leaves should be further explored as a potential source of natural antioxidants in certain food applications and for the cosmetic and pharmaceutical industries.

Related Topics
Life Sciences Agricultural and Biological Sciences Agronomy and Crop Science
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