Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4513640 | Industrial Crops and Products | 2013 | 5 Pages |
Amide compounds are the major bioactive substances in black pepper. Black pepper extracts were fractionated by high-speed countercurrent chromatography resulting in three compounds: dehydropipernonaline, piperine and (2E,4E,12Z)-N-isobutyl-octadecatrienamide, and four fractions which were separated by preparative HPLC to yield ten purified compounds: retrofractamide-A, (2E,4E)-N-isobutyl-decadienamide, retrofractamide-C, piperoleine A, dehydropiperoleine A, retrofractamide-B, piperoleine B, pipernonaline, pipercyclobutanamides A and (2E,4E,14Z)-N-isobutyleicosa-2,4,14-trienamide as identified by ESI-MS and NMR analysis. The results of the present study indicated that HSCCC is an effective technique for the preparation of bioactive amide compounds from black pepper.
► A good solvent system of HSCCC was selected to fractionate black pepper extract. ► HSCCC separation resulted in three individual amide compounds with hundred-mg scale. ► 13 individual amide compounds were harvested from HSCCC and HPLC separation.