| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4513765 | Industrial Crops and Products | 2013 | 11 Pages |
The aim of this study was to assess the contents of indigo's bioactive compounds, its antioxidant and anticancer activities in acetone, hexane and DMSO extracts and to compare the overall bioactivity with another more used medicinal plant named prolipid. It was found that the contents of the bioactive compounds in the studied extracts from different parts of indigo plant varied (P < 0.05): the significantly highest content of polyphenols and flavonoids was in DMSO extract of prolipid, flavanols – in acetone extract of brown seeds (P < 0.05 in both cases) and tannins – in DMSO extract of green leaves, but not significantly (P > 0.05). Also the level of antioxidant activity was different: the highest antioxidant activity of all studied samples was in prolipid: according to ABTS, FRAP and CUPRAC tests in DMSO extract (P < 0.05 in all 3 cases), and only in acetone extract according to DPPH was not significant (P > 0.05). The correlations between polyphenol compounds and the antioxidant activities were relatively high. DPPH kinetic measurements were used to compare and distinguish the antiradical activity among indigo extracts by multivariate analysis. The FT-IR spectroscopy evaluated the presence of polyphenols. The interaction between DMSO polyphenol extracts of indigo plant and BSA showed that indigo has a strong ability as other medicinal plants such as prolipid to quench the intrinsic fluorescence of BSA by forming complexes and was measured by 3-dimensional fluorescence (3D-FL). The highest anticancer activity was in prolipid in concentrations of 800 μg/mL against Calu-6, following by indigo brown leaves. In conclusion, organic extracts of indigo brown leaves were analyzed for their antioxidant and anticancer activities and compared with prolipid, using polyphenols composition, antioxidant activities and fluorescence properties. The indigo ability to quench the intrinsic fluorescence of BSA, relatively high content of phenolic compounds and anticancer properties can be used as medicinal plant.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Indigo plant is widely used as a dye, but few data known as a medicinal plant. ► Chemical composition and biological effects of this plant were investigated. ► Indigo leaves had high levels of phenolics, flavonoids, antioxidant activity. ► Indigo extract is potential agent against human cancer cells Calu-6 and SNU-601. ► Based on these properties indigo can be used as a medicinal plant.
