Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4516319 | Journal of Cereal Science | 2010 | 6 Pages |
Abstract
Proteomic techniques were applied for the investigation of “avenin-like protein type-B” in tetraploid wheat. Protein was extracted from endosperm, according to a classical chromatographic procedure and its identity confirmed by liquid chromatography-tandem mass spectrometry analysis. The characterization of avenin-like protein type-B was in good agreement with the gene-derived sequence, with the exception of glutamine as N-terminus. The presence in the glutenin extract and the high number of cysteine residues suggested that avenin-like protein type-B is integrated into gluten polymers via inter-chain disulphide bonds. This raises the question of whether the protein could play a role in determining the functional properties of gluten.
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Authors
Salvatore De Caro, Pasquale Ferranti, Francesco Addeo, Gianfranco Mamone,