Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4516423 | Journal of Cereal Science | 2008 | 7 Pages |
Abstract
B-Type avenin-like genes and proteins were characterized in 23 species of Triticeae. Southern blot analysis showed that the avenin-like genes belong to a multigene family. RT-PCR showed expression only in developing endosperms of wheat and related species, between 3 and 22 DPA (days post anthesis) with a peak between 11 and 15 DPA in wheat. The encoded proteins are cysteine-rich, containing 18–19 cysteine residues. An avenin-like protein from wheat was expressed in Escherichia coli, purified and used to raise polyclonal antibodies. These antibodies were used to detect b-type avenin-like proteins in endosperms of wheat and related species by western blotting.
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Authors
Peng Chen, Changdong Wang, Kexiu Li, Junli Chang, Yuesheng Wang, Guangxiao Yang, Peter R. Shewry, Guangyuan He,