| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4517692 | Postharvest Biology and Technology | 2016 | 9 Pages |
•Increases in DcETR1 transcript result in the recovery of ethylene sensitivity.•Ethylene-induced reduction in DcETR1 transcripts are completely suppressed by 1-MCP.•Transcripts of DcETR1 fluctuate periodically in association with petal senescence.•Increases in DcETR1 transcripts represent the appearance of new active receptors.•Ethylene binding to new receptors is prevented by successive treatment of 1-MCP.
In ethylene-sensitive plants, such as carnation, ethylene perception is considered an indispensable requirement to initiate and perpetuate the ethylene-mediated senescence program. Ethylene binding antagonists, such as 1-methylcyclopropene (1-MCP) compete for ethylene binding and block the hormone signaling. Despite its antagonistic propensity, plants treated with 1-MCP often recover sensitivity to ethylene post-treatment. Here we demonstrate that increases in the transcript level of the ethylene receptor DcETR1 results in the recovery of ethylene sensitivity in carnation petals treated with 1-MCP. This study reveals that the ethylene-induced reduction in mRNA levels of DcETR1 and DcCTR1 is completely suppressed by 1-MCP, and that the transcripts fluctuate periodically in association with petal senescence and ethylene biosynthesis. The results suggest that the periodic increase in receptor transcript may represent the appearance of new active receptors leading to renewed sensitivity to ethylene after treatment with 1-MCP. While ethylene sensitivity is temporarily blocked by 1-MCP, ethylene binding to new receptors is completely prevented by successive treatment of 1-MCP prior to recovery of ethylene-sensitivity, resulting in repression of petal senescence.
