| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4518280 | Postharvest Biology and Technology | 2014 | 8 Pages |
•A curative activity of heat (HT) was found on peach inoculated with M. laxa 48 h before treatment.•Cell wall genes involved in ripening (β-GAL, PL, PG, PME) showed a general decrease in expression in HT fruit as compared to the control.•ROS scavenging genes and PAL, CHI, HSP70 increased their expression levels in HT samples as compared untreated fruit.•Each analyzed gene proved to be differentially expressed following heat treatment.
Recent results on hot water as an alternative treatment open a new perspective in disease incidence reduction. In the present work peach fruit were wounded, inoculated with conidia of Monilinia laxa and 15 min, 3, 6, 12, 24 and 48 h after inoculation treated by dipping in hot water (HT) at 60 °C for 20 s. The effect of heat treatment on some cell wall genes involved in ripening such as β-galactosidase (β-GAL), pectin lyase (PL), polygalacturonase (PG) and pectin methyl esterase (PME), was analyzed by qRT-PCR. The expression levels of defense related genes, phenylalanine ammonia lyase (PAL) and chitinase (CHI), heat stress-related genes such as heat shock proteins 70 and 90 (HSP70, HSP90), and reactive oxygen species (ROS) scavenging genes were also evaluated by qRT-PCR. A 100% disease incidence reduction, as compared to untreated fruit, was obtained by treating 6 and 12 h after inoculation. Moreover, brown rot was inhibited by 85.7% when fruit were heat-treated 48 h after inoculation. The expression levels of cell wall genes (β-GAL, PL, PG and PME) showed a general decrease in HT fruit as compared to the control, whereas PAL, CHI, HSP70 and ROS-scavenging genes increased their expression level in HT samples with respect to the untreated ones. Our results show a curative activity of heat on peach inoculated with M. laxa 48 h before treatment. Each analyzed gene proved to be differentially expressed following heat treatment.
