Nematicidal effect of volatiles produced by Trichoderma sp.

Trichoderma is an important biocontrol agent that produces metabolites harmful to nematodes. We investigated the volatile organic compounds (VOCs) of Trichoderma sp. YMF 1.00416 and examined their abilities to kill nematodes. Chemical investigations of the VOCs from this strain led to the isolation and identification of three metabolites: a new compound, 1β-vinylcyclopentane-1α,3α-diol (1) and two known metabolites, 6-pentyl-2H-pyran-2-one (2) and 4-(2-hydroxyethyl)phenol (3). Nematicidal activity assays showed that compound 2 was nematicidal, and killed > 85% of Panagrellus redivivus, Caenorhabditis elegans, and Bursaphelenchus xylophilus in 48 h at 200 mg/L in a 2 mL vial. Our results will help identify new nematicides.

Graphical abstractThe fungus Trichoderma sp. YMF 1.00416 has been found to produce volatile organic compounds (VOC) to kill nematodes in a three-compartmented petri dish and a vial experiment. The nematicidal volatile organic compound 6-pentyl-2H-pyran-2-one was obtained from the stain.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Trichoderma sp. YMF 1.00416 produced compounds to kill nematodes. ► A new compound 1β-vinylcyclopentane-1α,3α-diol was obtained. ► Compound 6-pentyl-2H-pyran-2-one was a nematicidal volatile compound.