| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4524465 | Journal of Asia-Pacific Entomology | 2015 | 9 Pages |
•Two trehalase genes (HaTreh-1 and HaTreh-2) from H. armigera were cloned.•The bioinformation of HaTrehs was analyzed.•Multiple sequence alignment and phylogenetic trees of Trehs were built.•The expression patterns in various tissues were analyzed.•The expression patterns during developmental process were analyzed.
Trehalase plays a significant role in various physiological processes in insects. In this study, we cloned and characterized a soluble trehalase gene (HaTreh-1) and a membrane-bound trehalase gene (HaTreh-2) from Helicoverpa armigera, a serious polyphagous pest of crops. HaTreh-1 contained an open reading frame of 1716 bp that encodes a protein of 572 amino acids. HaTreh-2 has an open reading frame of 1938 bp, encoding a protein of 646 amino acids. Sequence alignment and phylogenetic analysis of the two putative proteins revealed that HaTreh-1 and HaTreh-2 belong to soluble and membrane-bound trehalase groups, respectively. Quantitative real-time PCR (qPCR) analyses of the spatiotemporal expression pattern of HaTreh in H. armigera revealed that HaTreh-1 was expressed mainly in the midgut, with lower expression in the integument and head, Malpighian tubules, trachea, and fat body. The expression levels of HaTreh-2 were detected in all 6 tissues, and HaTreh-2 was mainly expressed in the midgut and head. Expression of HaTreh-1 was higher throughout the larval stages, but lower on days 1 and 2 of the pupal stage. Expression of HaTreh-2 was higher during the 4th- and 5th-instar larval stages. Taken together, these results suggest that HaTreh-1 and HaTreh-2 have different functions in various developmental stages and tissues.
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