Article ID Journal Published Year Pages File Type
4524672 Journal of Asia-Pacific Entomology 2015 5 Pages PDF
Abstract

•SeGBPm expression in Sf9 insect cells decreased cell viability.•SeGBPm expression in Sf9 insect cells induced apoptotic cell death.•Recombinant AcNPV expressing SeGBPm decreased the body weights of S. exigua larvae.•AcNPV-SeGBPm expressing recombinant SeGBPm reduced LT50 by approximately 25%.

Insect cytokine growth-blocking peptides (GBPs) play functional roles in growth regulation and the innate immune response. Moreover, high concentrations of GBPs significantly induce larval morphological abnormalities and lethality. In the present study, a recombinant AcNPV, AcNPV-SeGBPm, expressing a growth-blocking peptide from the beet armyworm Spodoptera exigua (SeGBP) under the control of a polyhedrin promoter was constructed. Western blot analysis indicated that the mature peptide of SeGBP (SeGBPm) is expressed as a 3-kDa peptide in recombinant baculovirus-infected Sf9 insect cells. SeGBPm expression in Sf9 insect cells decreased cell viability significantly and induced apoptotic cell death. In addition, the body weights of S. exigua larvae inoculated with AcNPV-SeGBPm decreased significantly compared to those of larvae inoculated with wild-type AcNPV. Moreover, AcNPV-SeGBPm improved the rate of larvae killing (LT50) by approximately 1 day compared to wild-type AcNPV. Taken together, the obtained data demonstrate the great potential for future investigations of growth-blocking peptides for use as biopesticides.

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Life Sciences Agricultural and Biological Sciences Animal Science and Zoology
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