Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4528962 | Aquatic Toxicology | 2016 | 11 Pages |
•Ammonia exposure increases (Na+,K+)-, V(H+)-ATPase and antioxidant enzyme activities.•Relative expression of V(H+)-ATPase mRNA is increased by ammonia exposure.•ATPase distribution in gill ionocytes is unaffected by ammonia exposure.•Total hemolymph hemocyte count is decreased by ammonia exposure.
We evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na+,K+)- and V(H+)-ATPase activities and on their subunit expressions in gills of the diadromous freshwater shrimp Macrobrachium amazonicum. Specific (Na+,K+)- and V(H+)-ATPase activities increased roughly 1.5- to 2-fold, respectively, after exposure to 2.0 mmol L−1 TAN. Quantitative RT-PCR analyses revealed a 2.5-fold increase in V(H+)-ATPase B subunit mRNA expression while (Na+,K+)-ATPase α-subunit expression was unchanged. Immunohistochemical analyses of the gill lamellae located the (Na+,K+)-ATPase throughout the intralamellar septal cells, independently of TAN concentration, while the V(H+)-ATPase was located in both the apical pillar cell flanges and pillar cell bodies. Systemic stress parameters like total hemocyte count decreased by 30% after exposure to 2.0 mmol L−1 TAN, accompanied by increased activities of the oxidative stress enzymes superoxide dismutase, glutathione reductase and glucose-6-phosphate dehydrogenase in the gills. The stress responses of M. amazonicum to elevated TAN include increases in gill (Na+,K+)- and V(H+)-ATPase activities that are accompanied by changes in oxidative stress enzyme activities, immune system effects and an increase in gill V(H+)-ATPase gene expression. These findings likely underpin physiological effects in a crustacean like M. amazonicum that exploits multiple ecosystems during its life cycle, as well as under culture conditions that may significantly impact shrimp production by the aquaculture industry.