Multibiomarker responses upon exposure to tetrabromobisphenol A in the freshwater fish Carassius auratus

Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant. It has been released into aquatic environments, where it is toxic to aquatic organisms. In the present study, five enzymes, glutathione-S-transferase (GST), aspartate aminotransferase (AST), alanine aminotransferase (ALT), UDP-glucuronosyltransferase (UDPGT), and the antioxidant enzyme glutathione reductase (GR) in serum and liver of crucian carp (Carassius auratus) were selected for screening. These enzymes may be suitable for use as early warning indicators of chronic TBBPA exposure. UDPGT, AST, ALT, and GR activities in serum were found to be as more sensitive to TBBPA as those of the liver. When the concentration of TBBPA exceeded 0.50-0.71 mg/L for an exposure period of 32 days, GST, AST, ALT, and UDPGT activities cannot be restored to normal levels, suggesting that fish exposed to TBBPA above this threshold may incur irreversible damage. The activities of AST, ALT, and GR increased more significantly than GST and UDPGT at the lowest concentration of 0.35 mg/L. AST showed the strongest activity with respect to toxic kinetics, followed by ALT and GR. This remained true from day 4 of exposure to TBBPA to day 32. However, GR showed the clearest and most significant dose-effect relationship. This shows that each of these three enzymes can be used as a biomarker for early warning applications focusing on TBBPA pollution. AST and ALT are suitable for use in conventional monitoring of water quality in areas at risk for TBBPA pollution, and GR is more suitable for use in burst TBBPA pollution accidents where GR activity in fish would change with the TBBPA concentration of the flowing water.