Haematological and ion regulatory effects of nitrite in the air-breathing snakehead fish Channa striata

The tolerance and effects of nitrite on ion balance and haematology were investigated in the striped snakehead, Channa striata Bloch 1793, which is an air-breathing fish with reduced gills of importance for aquaculture in South East Asia. C. striata was nitrite tolerant with a 96 h LC50 of 4.7 mM. Effects of sub-lethal exposures to nitrite (0 mM, 1.4 mM, and 3.0 mM) were determined during a 7-day exposure period. Plasma nitrite increased, but the internal concentration remained well below ambient levels. Extracellular nitrate rose by several mM, indicating that a large proportion of the nitrite taken up was converted to nitrate. Nitrite reacted with erythrocyte haemoglobin (Hb) causing methaemoglobin (metHb) to increase to 30% and nitrosylhaemoglobin (HbNO) to increase to 10% of total Hb. Both metHb and HbNO stabilised after 4 days, and functional Hb levels accordingly never fell below 60% of total Hb. Haematocrit and total Hb were unaffected by nitrite. Although the effects of nitrite exposure seemed minor in terms of plasma nitrite and metHb increases, ion balance was strongly affected. In the high exposure group, total osmolality decreased from 320 mOsm to 260 mOsm, and plasma sodium from 150 mM to 120 mM, while plasma chloride fell from 105 mM to 60 mM and plasma bicarbonate rose from 12 mM in controls to 20 mM in exposed fish. The extreme changes in ion balance in C. striata are different from the response reported in other fish, and further studies are needed to investigate the mechanism behind the observed changes in regulation.

► Channa striata is tolerant to nitrite with a 96 h LC50 of 4.7 mM. ► Exposure to 3 mM nitrite reduces total osmolality and sodium with 20%. ► Exposure to 3 mM nitrite causes a 40% reduction in chloride. ► Despite exposure to 3 mM nitrite, methaemoglobin levels does not exceed 40%. ► The effects observed in C. striata are different than reported for other fish.