Article ID Journal Published Year Pages File Type
4530720 Aquatic Toxicology 2008 7 Pages PDF
Abstract
The objective of this work was to analyze oxidative metabolism in Mya arenaria. Total Fe content in M. arenaria collected in the German Wadden Sea was 1.9 ± 0.7, 0.7 ± 0.1 and 0.17 ± 0.01 nmol/mg fresh weight (FW), in digestive glands (DG), mantle and gills, respectively. Labile Fe pool, assessed by electronic paramagnetic resonance (EPR), was 146 ± 10 pmol/mg FW, and by the fluorescence method employing calcein it was 118 ± 9 pmol/mg FW. The lipid radical content in the DG, assessed by EPR, was 27 ± 7 pmol/mg FW, and the thiobarbituric reactive substances content amounted to 57 ± 8 pmol/mg FW. Ascorbyl radical (A) content, assessed by quantification of EPR signals, was 0.04 ± 0.01 pmol/mg FW, and the ascorbate content (AH−) was 478 ± 12 pmol/mg FW. The ratio A/AH− was (8 ± 1) × 10−5 AU, suggesting a minimum oxidative stress even under physiological conditions, presumably depending on basal metabolic functions. The content of nitric oxide (NO), assessed by EPR, was 99 ± 3 pmol/mg FW. The generation rate of NO by nitric oxide synthase-like activity (NOS-like) was assayed as NO production detected by EPR in the presence of l-arginine and NADPH, and was 3.16 ± 0.06 pmol/(mg FW min). The data presented here document the detectable presence of highly reactive species in M. arenaria.
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Life Sciences Agricultural and Biological Sciences Aquatic Science
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