| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4545555 | Harmful Algae | 2012 | 9 Pages |
The unicellular alga Prymnesium parvum has been responsible for toxic incidents with severe ecological impacts in many parts of the world, and causes massive fish kills worldwide. Recently the haptophyte microalgae have caused water-bloom (4.3 × 104 cells ml−1) in 6 fish ponds with high conductivity in Hungary, and caused fish mortality with typical symptoms. Toxicity of P. parvum from water samples was quantified by the assay of the influence of its cell-free filtrates on haemolysis (346 ± 42.2) and in fish and daphnia toxicity tests. High amount of proteases in P. parvum containing waterbloom samples were detected with the help of activity gel electrophoresis. The proteases of investigated P. parvum samples (125–18 kDa) showed high gelatinolytic activity and some of them showed sensitivity to EDTA (inhibitors of metalloproteases) and to PMSF (inhibitors of serine proteases).
► Prymnesium parvum is currently present in Hungary and causes fishkills in semi-soda lakes. ► Besides the haemolytic activity we detected high protease activities in some collected bloom samples and isolated P. parvum strains. ► The proteases of investigated P. parvum samples (125–18 kDa) showed high gelatinolytic activity and some of them showed sensitivity to EDTA (inhibitors of metalloproteases) and to PMSF (inhibitors of serine proteases). ► The detected numerous proteases may be related to mixotrophy of P. parvum and could enhance its toxicity and haemolytic activity.
