Genetic algorithm-based medium optimization for a toxic dinoflagellate microalga

A genetic algorithm stochastic search strategy was used to optimize the culture medium for producing the toxic marine dinoflagellate microalga Protoceratium reticulatum. The optimized medium contained 26 different components (macronutrients, trace elements, vitamins). The use of this medium allowed a 60% enhancement in the final cell concentration relative to the control culture based on L1 medium. The final titer of yessotoxins was improved by 40% relative to the control medium.

► This work uses genetic algorithm based experimentation to identify an optimal medium for a model toxic dinoflagellate that is commonly associated with the development of “red tides”. ► The new medium formulation is shown to be substantially superior to the L1-medium that is typically used for culturing dinoflagellates. ► Suitable culture media formulated specifically for dinoflagellates will facilitate their mass culture for isolation of marine toxins and other bioactives of potential environmental and commercial significance.