Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4545939 | Harmful Algae | 2009 | 7 Pages |
Abstract
The frequent occurrence of harmful algal blooms (HABs) is a pressing topic in marine research. An integrated sandwich hybridization and nuclease protection assay was established to qualitatively and quantitatively detect 12 harmful algal species. This method demonstrated good reliability, specificity and accuracy for analyzing samples from individual and mixed cultures, as well as field collection, and cell volumes were positively correlated to the slopes of calibration curves. The lowest quantitative detection limits were those concentrations observed during blooms; thus, this technique provides an efficient alternative to microscopy for rapid identification and quantitation of harmful algal species and could be routinely used to monitor phytoplankton in field surveys.
Keywords
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Agricultural and Biological Sciences
Aquatic Science
Authors
Yu Zhen, Tiezhu Mi, Zhigang Yu,