Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4554829 | Environmental and Experimental Botany | 2012 | 11 Pages |
Significant improvement of growth associated with increased, rather than decreased, uptake of Cu and Zn has been observed in poplar plants inoculated with Glomus spp. as compared with non-mycorrhizal plants. The beneficial effect exerted by these arbuscular mycorrhizal fungi (AMF) is likely to be controlled by specific gene expression patterns in the plant. Until now, however, very little is known about the transcriptional changes which occur in response to heavy metals (HMs) in mycorrhizal vs. non-mycorrhizal poplar plants. In order to identify such HM- and/or AMF-induced changes in leaves of white poplar (Populus alba L.) plants grown, in the greenhouse, on Cu- and Zn-polluted soil, the cDNA-Amplified Fragment Length Polymorphism (AFLP) approach was adopted, resulting in the identification of a number of new differentially regulated genes. Transcript derived fragments (TDFs) mostly belonged to stress-related functional categories of defence and secondary metabolism. Genes belonging to different functional categories, plus other genes known to be related to HM stress (metallothioneins, phytochelatin synthase, glutathione synthase, arginine decarboxylase), were analysed by quantitative (q)RT-PCR. Transcript levels were generally down-regulated, or unaffected, in polluted soil compared with controls, the main exceptions being phytochelatin synthase and clathrin, and strongly up-regulated in the presence of AMF, especially Glomus mosseae.
► We analysed the leaf transcriptome of a poplar clone in response to copper, zinc and Arbuscular Mycorrhizal Fungi (AMF). ► The transcriptome analysis methodology used was the cDNA-AFLP. ► This molecular technique has proven to be very effective to identify those genes associated with heavy metal stress response and to AMF colonization. ► The expression of the most interesting ones was evaluated by qRT-PCR.