Article ID Journal Published Year Pages File Type
4555714 Environmental and Experimental Botany 2007 9 Pages PDF
Abstract

Two separate objectives were considered in this study. We examined (1) internal conductance to CO2 (gi) and photosynthetic limitations in sun and shade leaves of 60-year-old Fagus sylvatica, and (2) whether free-air ozone fumigation affects gi and photosynthetic limitations. gi and photosynthetic limitations were estimated in situ from simultaneous measurements of gas exchange and chlorophyll fluorescence on attached sun and shade leaves of F. sylvatica. Trees were exposed to ambient air (1× O3) and air with twice the ambient ozone concentration (2× O3) in a free-air ozone canopy fumigation system in southern Germany (Kranzberg Forest). gi varied between 0.12 and 0.24 mol m−2 s−1 and decreased CO2 concentrations from intercellular spaces (Ci) to chloroplastic (Cc) by approximately 55 μmol mol−1. The maximum rate of carboxylation (Vcmax) was 22–39% lower when calculated on a Ci basis compared with a Cc basis. gi was approximately twice as large in sun leaves compared to shade leaves. Relationships among net photosynthesis, stomatal conductance and gi were very similar in sun and shade leaves. This proportional scaling meant that neither Ci nor Cc varied between sun and shade leaves. Rates of net photosynthesis and stomatal conductance were about 25% lower in the 2× O3 treatment compared with 1× O3, while Vcmax was unaffected. There was no evidence that gi was affected by ozone.

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