Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4555749 | Environmental and Experimental Botany | 2006 | 8 Pages |
Abstract
In our previous work, we described the defensive potential of a wound- and methyl jasmonate-inducible 87Â kDa tomato cystatin and its accumulation in a crystalline form. Here, we report the immunolocalization of this cysteine proteinase inhibitor in tomato leaf blade. Methyl jasmonate treated wild type plants showed accumulation of crystalline structures that were specifically and strongly stained with polyclonal antibodies against tomato cystatin. Crystalline cystatin was found in palisade and spongy parenchyma cells and immuno-gold electron microscopy analysis revealed that the crystals were compartmentalized in the cytosol. The same pattern in localization of cystatin was observed in transgenic tomato plants superexpressing prosystemin transgene. Our data showing the accumulation of cystatin in response to methyl jasmonate and in response to a overproduction of a wound signal corroborate the protective role of this inhibitor.
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Authors
Hérika C. Madureira, Maura Da Cunha, Tânia Jacinto,