| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4557508 | Journal of Invertebrate Pathology | 2016 | 4 Pages |
•Efficacy of Bacillus thuringiensis (Bt) proteins against Samia cynthia determined.•Cry1Aa, Cry1Ab, Cry1Ac proteins highly toxic to eri silkworm.•Castor transgenics harbouring Bt genes should be bioassayed for eri silkworm.
Ten purified crystal proteins of Bacillus thuringiensis (Bt) were tested at concentrations ranging from 2.93 to 3000 ng/cm2 for their toxicity to eri silkworm through protein paint bioassays using castor leaves. Based on LC50 values, Cry1Aa (2.6 ng/cm2) was highly toxic followed by Cry1Ac (29.3 ng/cm2) and Cry1Ab (68.7 ng/cm2). The Cry1Ca and Cry1Ea proteins were moderately toxic to eri silkworm larvae and resulted in 23% and 28% mortality, respectively at the highest concentration tested (3000 ng/cm2). Only reduction in larval weight was observed with Cry2Aa, Cry1Da and Cry9Aa proteins while Cry3Aa and Cry1Ba proteins were found to be nontoxic.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
