| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4566600 | Scientia Horticulturae | 2014 | 7 Pages |
•Polyembryonic masses of two date palm varieties were cryopreserved.•Droplet-vitrification (DV) led to 85.6–98.6% recovery after cryopreservation.•With DV, sucrose preculture slightly reduced recovery after liquid nitrogen exposure.•V cryo-plate produced 67.0–95.8% recovery after cryopreservation.•With V cryo-plate, sucrose preculture increased recovery after cryopreservation.
In this work we tested the efficiency of two techniques, droplet-vitrification (DV) and dehydration (D) cryo-plate, for cryopreservation of polyembryonic masses (PEMs) of two date palm varieties, Sokary and Sultany. With DV, recovery of non-precultured, cryopreserved PEMs was nil without treatment with the PVS2 vitrification solution, which contained 3.3 M glycerol + 2.4 M ethylene glycol + 0.4 M sucrose + 1.9 M dimethylsulfoxide. Following PVS2 treatments between 15 and 120 min, it was comprised between 90.9–98.6% and 85.6–88.0% for varieties Sokary and Sultany, respectively. Sucrose preculture (3 days, 0.5 M) led to 21.1% recovery of cryopreserved PEMs of variety Sokary only without PVS2 treatment, and slightly reduced recovery in all other experimental conditions with both varieties, compared with non-cryopreserved PEMs. Regrowth intensity of cryopreserved PEMs was generally lower with variety Sultany compared to variety Sokary. With the D cryo-plate technique, no recovery of cryopreserved PEMs was achieved without sucrose preculture. Sucrose preculture (3 days, 0.5 M) had a positive effect on recovery of cryopreserved PEMs. For variety Sokary, the highest recovery (92.0–95.8%) was achieved for desiccation periods between 60 and 120 min. Recovery was between 67.0 and 74.6% after desiccation periods of 90–120 min for variety Sultany. With the D cryo-plate technique, regrowth intensity of cryopreserved PEMs was higher with variety Sokary compared to variety Sultany.
