| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4567195 | Scientia Horticulturae | 2013 | 8 Pages |
A molecular marker associated with male fertility restoration was newly identified in onion in this study using random amplification of polymorphic DNA (RAPD) and bulk segregant analysis. A polymorphic amplicon from RAPD reaction was observed in DNA from fertile plants. The amplicon was designated as the RAPD marker OBC14.1000 and a 980-base pair (bp) nucleotide sequence was obtained. Genome walking was performed to obtain the flanking sequences of the OBC14.1000 marker to develop a co-dominant marker. The acquired sequences were a 2049-bp fragment linked to a male-fertile allele and a 1621-bp fragment linked to a male-sterile allele. Approximately 95% similarity was shown when the nucleotide sequences between fertile and sterile alleles were aligned. Using a polymorphic region containing a restriction site of AvaII in a fertile allele, the RAPD marker OBC14.1000 was successfully converted into a co-dominant CAPS marker and it was designated as ACms.1100. It appeared to be positioned much closer to the restorer-of-fertility locus, Ms, based on genotyping analysis as compared to other currently available markers. No putative homology was found to the newly identified ACms.1100 marker sequences through a Blast database search and the identification of sequences is still unknown.
► Molecular markers tightly linked to male fertility restoration were identified. ► The marker was designated as the RAPD marker OBC14.1000. ► Using DNA walking, the sequences flanking the RAPD marker were acquired. ► The CAPS marker was designated as the ACms.1100 which is codominant. ► No recombinant was observed in F2 and F3 progeny.
