| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4567741 | Scientia Horticulturae | 2012 | 4 Pages |
Abstract
⺠An improved method for preparing high molecular weight DNA from chrysanthemum was firstly developed. ⺠A considerable yield of HMW-DNA more than 2 Mb was achieved. ⺠The improved method utilizes a modified buffer system including 1% (v/v) β-mercaptoethanol, 4 mM spermidine and 6% (w/v) PVP-40 and a centrifugal speed of 1600 Ã g to extract and suspend HMW-DNA. ⺠No difference on DNA fragment size was observed with different lysis times. The HMW-DNA can be partially digested to 23-80 kb in size.
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Authors
Xinwei Guo, Xiuhai Zhang, Zhongyi Wu, Chang Luo, Xi Cheng, Conglin Huang,