| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4568090 | Scientia Horticulturae | 2011 | 6 Pages |
An efficient protocol to improve microspore embryogenesis and plant regeneration in Brassica rapa was established. The antiauxin p-chlorophenoxyisobutyric acid (PCIB) was used to enhance microspore embryogenesis and plant regeneration without an intervening callus phase. All the 4 tested genotypes responded positively to PCIB. The optimum concentration of PCIB application was found to be 40 μM in NLN-13 medium, which resulted in a 3.4- to 6.2-fold increase in the number of embryos (8.27–19.2 embryos per bud) and a 9.6-fold increase (21.33%) in the plant regeneration frequency in comparison with the controls. Heat-shock treatment by incubation at 35 °C for 1 day was more efficient in inducing embryogenesis in the 2 tested genotypes. The embryos, produced in NLN medium supplemented with 40 μM PCIB and transferred at the 21-day-old followed by a treatment at 4 °C for 5 days, reached the highest direct plant regeneration rate of 58.00%.
► Brassica rapa is an important leafy vegetable and has been widely planted in Asia. ► The microspore culture protocol was improved through adding PCIB to the induction medium. ► The frequency of microspore embryogenesis and plant regeneration was increased significantly. ► The whole process was faster than the traditional method.
