Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4568875 | Scientia Horticulturae | 2009 | 4 Pages |
Abstract
Multiple shoot formation from the plumular apices excised from mature embryos of cowpea cv. Akkiz was obtained after pulse treatment with 10Â mg/l BAP for 5 days followed by culture on MS medium containing 0.25, 0.50, 0.75 and 1.00, 1.25Â mg/l BAP - with or without 0.10Â mg/l NAA. Callus induction and shoot regeneration was recorded on all cultures containing BAP with or without NAA. However, inclusion of 0.1Â mg/l NAA had positive effect on callus diameter and shoot length. Maximum mean number of 7.11 shoots per explant were obtained on MS medium containing 1.00Â mg/l BAP. Longer shoots were recorded on MS medium containing various concentration of BAP+ 0.1Â mg/l NAA compared to those containing various concentrations of BAP singly. All shoots cultured on MS medium containing 1Â mg/l BAP were rooted on MS medium containing 0.50Â mg/l IBA. Rooted plants were acclimatized at room temprature in soil contained in pots. All plants flowered and set seeds in the greenhouse after 3 months.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Horticulture
Authors
Muhammad Aasim, Khalid Mahmood Khawar, Sebahattin Ãzcan,