| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4568881 | Scientia Horticulturae | 2009 | 5 Pages |
Abstract
A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.
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Authors
Milene Miranda Praça, Carlos Roberto Carvalho, Wellington Ronildo Clarindo,