Label-free quantitative proteomic analysis of inhibition of Xanthomonas axonopodis pv. citri by the novel bactericide Fubianezuofeng

•A comparative proteomic analysis was conducted in this study.•339 and 314 proteins regulated by Fubianezuofeng with 1/2 and 1 of MIC, respectively•Pyrimidine metabolism was a main pathway regulated by Fubianezuofeng.

To understand the antibacterial mechanism of the new bactericide 2-(methylsulfonyl)-5- (4-fluorobenzyl)-1, 3, 4-oxadiazole (Generic name: Fubianezuofeng), we performed label-free quantitative proteomics analysis of the response of Xanthomonas axonopodis pv. citri (Xac) strain 29-1 to Fubianezuofeng. A total of 1133 proteins were identified in the treatment and control groups. Upon treatment with the 1/2 minimum inhibitory concentration (MIC), 339 proteins were found to be differentially expressed (fold changes > 1.5, p < 0.05) with 99 upregulated and 240 down-regulated. In comparison, 314 proteins were differentially expressed (125 up-regulated, 189 down-regulated) at MIC. The differentially expressed proteins were enriched for those involved in the pyrimidine metabolic pathway. The results offer a complete view of the proteome changes in bacteria in response to Fubianezuofeng.

Graphical abstractIn this paper, label-free quantitative proteomics coupled with bioinformatics was used to analyze the mechanism underlying the response of Xac strain 29-1 to Fubianezuofeng, a novel bactericide. Pyrimidine metabolism was the most strongly enriched pathway among the proteins differentially expressed between treatment groups. The results offer a more complete view of the proteome changes in bacteria in response to Fubianezuofeng.Download high-res image (93KB)Download full-size image