Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5515882 | Plant Science | 2016 | 12 Pages |
â¢636 coldâshock responsive genes were identified in three wheat genotypes.â¢More upâ than downâregulated genes were identified in both freezingâsensitive and freezingâtolerant genotypes.â¢ABA and its metabolites responded oppositely to coldâshock in all lines.â¢ABAâsignalling might have a role in coldâshock response of the examined genotypes.â¢Genotypes had differences in signal informationâflow and stressâresponsive biological processes.
The effect of one-day cold-shock on the transcriptome and phytohormones (auxin, cytokinins, abscisic, jasmonic and salicylic acids) was characterised in freezing-sensitive (Chinese Spring), highly freezing-tolerant (Cheyenne) and moderately freezing-tolerant (Chinese Spring substituted with Cheyenne's 5A chromosome) wheat genotypes. Altogether, 636 differentially expressed genes responding to coldâshock were identified. Defence genes encoding LEA proteins, dehydrins, chaperons and other temperature-stress responsive proteins were upâregulated in a genotypeâindependent manner. Abscisic acid was up-regulated by cold accompanied by adherent expression of its metabolic genes. Data revealed the involvement of particular routes within ABA-dependent signalling in response to coldâshock in the examined genotypes. Coldâshock affected gene expression along carbohydrate metabolic pathways. In photosynthesis, coldâshock changed the expression of a number of genes in the same way as it was previously reported for ABA. Overrepresentation analysis of the differentially expressed genes supported the ABAâsignalling and carbohydrate metabolism results, and revealed some pronounced biological process GO categories associated with the coldâshock response of the genotypes. Protein network analysis indicated differences between the genotypes in the information flow along their signal perception and transduction, suggesting different biochemical and cellular strategies in their reaction to coldâshock.