Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5517551 | European Journal of Protistology | 2017 | 6 Pages |
This study investigated the prevalence of Cryptosporidium in farmed fur animals in the Czech Republic and Poland. A total of 480 faecal samples were collected from fur animals, including 300 American mink (Mustela vison), 60 silver foxes (Vulpes vulpes), 50 long-tailed chinchillas (Chinchilla lanigera), and 70 nutrias (Myocastor coypus), at 14 farms. Samples were examined for the presence of Cryptosporidium using microscopy (following aniline-carbol-methyl violet staining) and sequence analysis of PCR amplified products. Three mink and two chinchillas from two different farms tested positive for Cryptosporidium ubiquitum DNA. The presence of C. ubiquitum DNA was not associated with diarrhoea. Subtyping of C. ubiquitum isolates by sequence analysis of the 60-kDa glycoprotein gene showed that isolates belonged to the XIIa subtype family, which was previously restricted to humans and ruminants. This suggests that C. ubiquitum subtype XIIa has a broader host range than previously reported.