Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5520848 | The Crop Journal | 2017 | 6 Pages |
Abstract
Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agronomy and Crop Science
Authors
Congsheng Zhang, Changlin Liu, Jianfeng Weng, Beijiu Cheng, Fang Liu, Xinhai Li, Chuanxiao Xie,