Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5522585 | Stem Cell Research | 2017 | 4 Pages |
Abstract
SIX2-positive renal cells isolated from urine from a 51 year old male of African origin bearing the CYP2D6 *4/*17 variant were reprogrammed by nucleofection of a combination of two episomal-based plasmids omitting pathway (TGFβ, MEK and GSK3β) inhibition. The induced pluripotent stem cells (iPSCs) were characterized by immunocytochemistry, embryoid body formation, DNA-fingerprinting and karyotype analysis. Comparative transcriptome analyses with human embryonic stem cell lines H1 and H9 revealed a Pearson correlation of 0.9243 and 0.9619 respectively.
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Authors
Martina Bohndorf, Audrey Ncube, Lucas-Sebastian Spitzhorn, Jürgen Enczmann, Wasco Wruck, James Adjaye,