| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522588 | Stem Cell Research | 2017 | 4 Pages |
Abstract
Peripheral blood mononuclear cells were isolated and cultured to a pure pro-EBL population and reprogrammed using episomal plasmids. The pluripotency of transgene-free induced pluripotent stem cell (iPSC) line was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. The iPSC line showed normal karyotype. Peripheral blood is a non-invasive easy accessible cell source and combined with EBL outgrowth in vitro, a routine process obtaining sufficient amount of homogenous cells can be obtained within a week. Using episomal delivery, pro-EBLs can be reprogrammed in a transgene-free, cost effective system.
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Authors
Eszter Varga, Marten Hansen, Tatjana Wüst, Marieke von Lindern, Emile van den Akker,