| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522634 | Stem Cell Research | 2017 | 4 Pages |
Abstract
Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.
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Authors
Christina Paulitschek, Peggy Schulze-Matz, Julia Hesse, Timo Schmidt, Wasco Wruck, James Adjaye, Jürgen Schrader,