| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522701 | Stem Cell Research | 2017 | 4 Pages |
Abstract
Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
