Characterization of dermal fibroblast-derived iPSCs from a patient with high grade steatosis

Primary fibroblasts from a high grade steatosis patient were reprogrammed by transduction of retroviruses OCT4, SOX2, c-MYC and KLF4. IPSCs were characterized by immunocytochemistry, embryoid body-formation, DNA-fingerprint, karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation coefficient of 0.9287.Resource table.Name of stem cell constructS08InstitutionInstitute for Stem Cell Research and Regenerative MedicinePerson who created resourceJustyna JozefczukContact person and emailJames Adjaye, James.Adjaye@med.uni-duesseldorf.deDate archived/stock dateAugust 2012OriginPrimary human fibroblastsType of resourceBiological reagent: induced pluripotent stem cell (iPSC); derived from steatosis patients and a healthy individualSub-typeCell lineKey transcription factorsOCT4, SOX2, c-MYC and KLF4AuthenticationIdentity and purity of cell line confirmed (Figs. 1, 2)Link to related literature (direct URL links and full references)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672680/Information in public databasesNone