| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5522816 | Stem Cell Research | 2017 | 4 Pages |
Abstract
The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37 bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
