Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5538090 | Agriculture, Ecosystems & Environment | 2016 | 17 Pages |
Abstract
With the increase in the number of commercial applications and larger cultivation areas of genetically modified (GM) plants, their biosafety for soil microorganisms has become a controversial issue. The effects on the diversity and abundance of soil microorganisms are important components of evaluation of the biosafety risks of GM plants. So far, no definite conclusions have been drawn about whether GM plants can negatively affect soil microorganisms. In this review, we discuss the advances that have been made in recent years in the research into the effects of GM plants on soil microbial communities. It has been argued that foreign gene products that are released from the residue of GM plants into soil by root exudation may affect soil microbial communities. Moreover, foreign genes may change the genetic and functional properties of soil microorganisms via horizontal transfer. The advantages and disadvantages of various detection technologies-from classical culture-dependent methods to modern molecular protocols-are reviewed here. To accurately and comprehensively evaluate the effects of GM plants on microorganisms, we discuss the factors that should be considered in the assessment of risks of GM plants for soil microorganisms (e.g., foreign proteins, marker genes, plant varieties, and environmental factors), as well as the problems and prospects related to biosafety assessment platforms for GM plants.
Keywords
GM plantPRSVT-RFLPDGGEPLFAsNGSPapaya ringspot virusGFPAMVRFLPCaMVRT-PCRCFUsCLPPReal-time PCRARDRAARISANon-target effectphospholipid fatty acidsGenetically modifiedDenaturing gradient gel electrophoresisBacillus thuringiensisautomated ribosomal intergenic spacer analysisRibosomal Intergenic Spacer Analysisamplified ribosomal DNA restriction analysisNext generation sequencingMolecular techniqueEcological riskRisafluorescence in situ hybridizationterminal restriction fragment length polymorphismFishCommunity-level physiological profileSoil microorganismcolony-forming unitscauliflower mosaic virusAlfalfa mosaic virusgreen fluorescent proteinrestriction fragment length polymorphism
Related Topics
Life Sciences
Agricultural and Biological Sciences
Agronomy and Crop Science
Authors
Zheng-jun Guan, Shun-bao Lu, Yan-lin Huo, Zheng-Ping Guan, Biao Liu, Wei Wei,