Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5539252 | Aquaculture | 2017 | 29 Pages |
Abstract
The aim of this study was to evaluate the efficacy of essential oil of citronella, Cymbopogon nardus (EOC) as an anaesthetic for fish, using juvenile tambaqui Colossoma macropomum as a model. The times to induction and recovery after short-term anaesthesia were evaluated by concentration-response trials. Moreover, electrophysiological responses in muscle (EMG), electrocardiographic data (ECG), opercular beat intensity (OBI) and rate (OBR) were also evaluated. Juveniles were placed in aquaria containing six concentrations of EOC (200, 400, 600, 800, 1000 and 1600 μL Lâ 1) and the times of anaesthetic induction and recovery were registered. Sham control fish and fish exposed to ethanol-added water were used as controls. With the exception of the lowest concentration used (200 μL Lâ 1), deep anaesthesia was attained at all other subsequent concentrations, being the use of 600 μL Lâ 1 sufficient to promote rapid induction (< 3 min) and uneventful recovery (< 5 min) from deep anaesthesia; while the concentration of 200 μL Lâ 1 was sufficient to induce sedation. EOC elicited a conspicuous depression on muscle contraction power with loss of muscle tonus. Although transient cardiorespiratory depression occurred during deep anaesthesia, gradual increases in the amplitudes of the differences in potential and frequencies in EMG, OBI and ECG, corroborate the reversibility and smooth transition to normal swimming behaviour during recovery from anaesthesia with EOC.
Related Topics
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Agricultural and Biological Sciences
Aquatic Science
Authors
Luis André Luz Barbas, Moisés Hamoy, Vanessa Jóia de Mello, Ruan Paulo Melo Barbosa, Hildado Socorro Trindade de Lima, Marcelo Ferreira Torres, Luis Adriano Santos do Nascimento, Joyce Kelly do Rosário da Silva, EloÃsa Helena de Aguiar Andrade,