Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5542634 | Journal of Dairy Science | 2016 | 4 Pages |
Abstract
The refrigeration (4, 8, 24, or 48 d), freezing (1, 7, or 30 h), thawing (25, 40, or 60°C), and usage of preservatives (sodium azide, potassium dichromate, sodium thiocyanate, bronopol, and methanol), of raw bovine milk were investigated for β-lactamase activity. The β-lactamase activity in all samples was assessed by using the test strips, and the samples with the preservatives were further assessed for β-lactamase activity using the cylinder plate method. Results showed that the refrigeration, freezing, and thawing of raw bovine milk samples had no influence on the assay of the β-lactamase activity. The addition of sodium azide or potassium dichromate as a sample preservative failed the test of β-lactamase activity, whereas the addition of sodium thiocyanate, bronopol, or methanol had no influence on the test. In conclusion, sodium azide and potassium dichromate were not suitable preservatives for assessing β-lactamase activity in raw milk when the cylinder plate method was used.
Keywords
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Animal Science and Zoology
Authors
Y.D. Zhang, N. Zheng, X.Y. Qu, S.L. Li, J.H. Yang, S.G. Zhao, Yasmeen Saeed, L. Meng, J.Q. Wang,