Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5543918 | Research in Veterinary Science | 2017 | 28 Pages |
Abstract
Pharmacokinetic and pharmacodynamic (PK/PD) properties of the angiotensin-converting enzyme inhibitor (ACEI) benazeprilat have not been evaluated in horses. This study was designed to establish PK profiles for benazepril and benazeprilat after intravenous (IV) and oral (PO) administration of benazepril using a PK/PD model. This study also aims to determine the effects of benazeprilat on serum angiotensin converting enzyme (ACE), selecting the most appropriate dose that suppresses ACE activity. Six healthy horses in a crossover design received IV benazepril at 0.50Â mg/kg and PO at doses 0 (placebo), 0.25, 0.50 and 1.00Â mg/kg. Blood pressures (BP) were measured and blood samples were obtained at different times in order to measure serum drug concentrations and serum ACE activity, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and spectrophotometry, respectively. Systemic bioavailability of benazeprilat after PO benazepril was 3-4%. Maximum ACE inhibitions from baseline were 99.63% (IV benazepril), 6.77% (placebo) and 78.91%, 85.74% and 89.51% (for the three PO benazepril doses). Significant differences in BP were not found. Although oral availability was low, benazeprilat 1.00Â mg/kg, reached sufficient serum concentrations to induce long lasting serum ACE inhibitions (between 88 and 50%) for the first 48Â h. Additional research on benazepril administration in equine patients is indicated.
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Authors
Juan Manuel Serrano-RodrÃguez, Manuel Gómez-DÃez, MarÃa Esgueva, Cristina Castejón-Riber, Antonio Mena-Bravo, Feliciano Priego-Capote, Nahúm Ayala, Juan Manuel Serrano Caballero, Ana Muñoz,