Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5544740 | Veterinary Immunology and Immunopathology | 2017 | 12 Pages |
Abstract
Different species of camelids play an important role in the epidemiology of various emerging infectious diseases such as Middle East respiratory syndrome. For precise investigations of the immunopathogenesis in these host species, appropriate immunohistochemical markers are highly needed in order to phenotype distinct immune cells populations in camelids. So far, specific immunohistochemical markers for camelid immune cells are rarely commercially available, and cross-reactivity studies are restricted to the use of frozen dromedary tissues. To bridge this gap, 14 commercially available primary antibodies were tested for their suitability to demonstrate immune cell populations on formalin fixed paraffin-embedded (FFPE) tissue sections of dromedaries, Bactrian camels, llamas, and alpacas in the present study. Out of these, 9 antibodies directed against CD3, CD20, CD79α, HLA-DR, Iba-1, myeloid/histiocyte antigen, CD204, CD208, and CD68 antigen exhibited distinct immunoreaction patterns to certain camelid immune cell subsets. The distribution of these antigens was comparatively evaluated in different anatomical compartments of thymus, spleen, mesenteric, and tracheobronchial lymph nodes. The presented results will provide a basis for further investigations in camelids, especially with respect to the role of the immune response in certain infectious diseases, which harbor a considerable risk to spill over to other species.
Keywords
IgGPAX-5DC(s)GARGAMDLTMUM1MERS-CoVRARFOXP3PBSDromedaryMiddle East respiratory syndrome coronavirusH&EIba-1FFPEAlpacahuman leucocyte antigenHLAImmunohistochemistryIHCimmunoglobulin Gforkhead box P3cluster of differentiationRoom temperatureB cellsT cellsdendritic cell(s)Camelidsperiarteriolar lymphoid sheathformalin-fixed paraffin-embeddedPhosphate buffered salineLlamaMacrophagesionized calcium-binding adapter molecule 1MonoclonalHematoxylin and EosinPALSPolyclonal
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Authors
Ann-Kathrin Uhde, Annika Lehmbecker, Wolfgang Baumgärtner, Ingo Spitzbarth,