Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5544876 | The Veterinary Journal | 2017 | 23 Pages |
Abstract
Four of 15 HSCC tested positive for EcPV2 DNA and harboured known or novel genetic variants of E6, E7, E2 and the LCR. Eighteen of 35 sample extracts including 3/4 smegma samples scored positive by IC/PCR, suggesting that about 51% of tested extracts harboured virions. E6/E2 qPCR from tumour DNA revealed E2/E6 copies/cell ranging between <1 (E2; E6) and 797 (E2) or 1434 (E6). IC/PCR-positive smegma samples contained higher E2 and E6 copy numbers, ranging between 1490 and 4.95Â ÃÂ 105 (E2) or 2227 and 8.54Â ÃÂ 105 (E6) copies/cell. Together with IC/PCR results, this finding suggests that smegma can serve as a rich EcPV2 reservoir. HSCCs harboured significantly lower viral DNA amounts (<1-25 copies/cell) than most genital tumour and smegma DNA isolates. The majority of samples contained more E6 than E2 DNA, with E6:E2 ratios ranging between 0.88 and 4.12. Although not statistically significant (PÂ >Â 0.05), this finding suggests that EcPV2 can integrate into the equine host cell genome.
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Authors
S. Sykora, C. Jindra, M. Hofer, R. Steinborn, S. Brandt,