Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5585461 | Comptes Rendus Biologies | 2017 | 12 Pages |
Abstract
The plants belonging to the Ericaceae family are morphologically diverse and widely distributed groups of plants. They are typically found in soil with naturally poor nutrient status. The objective of the current study was to identify cultivable mycobionts from roots of nine species of Ericaceae (Calluna vulgaris, Erica arborea, Erica australis, Erica umbellate, Erica scoparia, Erica multiflora, Arbutus unedo, Vaccinium myrtillus, and Vaccinium corymbosum). The sequencing approach was used to amplify the Internal Transcribed Spacer (ITS) region. Results from the phylogenetic analysis of ITS sequences stored in the Genbank confirmed that most of strains (78) were ascomycetes, 16 of these were closely related to Phialocephala spp, 12 were closely related to Helotiales spp and 6 belonged to various unidentified ericoid mycorrhizal fungal endophytes. Although the isolation frequencies differ sharply according to regions and ericaceous species, Helotiales was the most frequently encountered order from the diverse assemblage of associated fungi (46.15%), especially associated with C. vulgaris (19.23%) and V. myrtillus (6.41%), mostly present in the Loge (L) and Mellousa region (M). Moreover, multiple correspondence analysis (MCA) showed three distinct groups connecting fungal order to ericaceous species in different regions.
Keywords
tris acetate EDTAEricaceous shrubsMCAEricoid mycorrhizal fungiITSHelotialesEricoid mycorrhizaeDSEPDAPACMMNERMSASRDNADNAInternal transcribed spacerSTATStatisticsBLAST, basic local alignment search tooldeoxyribonucleic acidribosomal deoxyribonucleic acidDark septate endophytesBlastMultiple correspondence analysisTAESodium hypochloritepotato dextrose agarStatistical Analysis SystemMusclePhosphorusmultiple sequence comparison by log-expectationNitrogenpolymerase chain reactionPCRPotassiumNeighbor joining
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Authors
Ahlam Hamim, Lucie Miché, Ahmed Douaik, Rachid Mrabet, Ahmed Ouhammou, Robin Duponnois, Mohamed Hafidi,