Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5588896 | Agri Gene | 2016 | 9 Pages |
â¢Rice and maize weevil mitochondrial genomes were assembled from next generation sequence.â¢Pronounced non-coding intergenic regions with tandem repeat arraysâ¢Non-synonymous substitution mutations found in the two weevil mitogenomes could serve as ideal markers.â¢Sequence data are good resources for evolutionary relationship among Coleoptera.
Complete mitochondrial genome sequences were assembled for the rice weevil, Sitophilus oryzae (17,602Â bp) and the maize weevil, S. zeamais (18,105Â bp; Coleoptera: Curculionidae: Dryophthorinae), which encode 13 protein coding genes (PCGs), 22 transfer RNAs, and 2 ribosomal RNAs that is typical among Animalia. The AÂ +Â T-rich control regions of S. oryzae (2818Â bp) and S. zeamais (2832Â bp) are the longest described to date among curculionoidea (weevils). Additionally, non-coding intergenic regions have increased in size due to expansion of tandem repeat arrays, but is more pronounced in S. zeamais (606Â bp) compared to S. oryzae (109Â bp). A total of 69 and 22 substitution mutations were found among reads from S. oryzae and S. zeamais, out of which 68 and 2 were predicted in the PCGs respectively, with a majority in NADH Dehydrogenase subunit I. Phylogenetic analyses of coleopteran insects based upon full mitogenomes PCG sequence supported the existence of Curculionoidea and Tenebrionoidea as a monophyletic group, whereas the Cucujoidea and Elateroidea remain paraphyletic. The mitogenomes of these two Sitophilus species provide insight into short-term evolutionary relationships among curculionid beetles, and provide resources for the potential deciphering of more broad systematic questions in the Order Coleoptera.