Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5588905 | Agri Gene | 2017 | 8 Pages |
Abstract
Type I interferon (IFN) is a key cytokine of innate immune response for viral infection in teleost fish. In present study we report the identification of a type I IFN gene (AjIFN) from Japanese eel (Anguilla japonica) and provide evidence that AjIFN has been involved in Japanese eel host response against not only the virus but also the bacterial infection. The full-length cDNA of AjIFN (826Â bp) has an ORF of 531Â bp. The analysis of NCBI CDD showed that the AjIFN protein had the typical conserved domains, including IFabd conserved domain, and two conserved cysteine residues potentially forming disulphide bridges. Based on the phylogenetic analysis, AjIFN was classified into type I IFNs families belonging to the subgroup-a of the 2Cys group. In vivo, the AjIFN expressions in liver and kidney were induced following injection with LPS, the viral mimic poly I:C, and Aeromonas hydrophila infection. In vitro, the AjIFN transcripts of Japanese eel liver cells were enhanced by LPS, poly I:C and CpG-DNA stimulation and no change of the expression level was found post PGN treatment. Following A. hydrophila infection, the low concentration of 1Â ÃÂ 106Â cfu/mL failed to induce the expression of AjIFN whereas the concentration of 1Â ÃÂ 107Â cfu/mL and 1Â ÃÂ 108Â cfu/mL successfully induced the expression of AjIFN. These results collectively suggested AjIFN is an inducible gene possibly involved in Japanese eel defense against viral and bacterial infection.
Keywords
UTRmRNAORFAeromonas hydrophilaPAMPsRLRsqRT-PCRPGNTSBTLRLPScDNADNA complementary to RNAmessenger RNAAnguilla japonicainterferonType I interferonIFNmRNA expressionrapid amplification of cDNA endsToll-like receptorTryptone soya brothopen reading framelipopolysaccharideRaceuntranslated regionquantitative real-time polymerase chain reactionpathogen associated molecular patternsPoly I:Cpolyinosinic-polycytidylic acidPeptidoglycanRIG-I-like receptors
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Authors
Jianjun Feng, Peng Lin, Yilei Wang, Songlin Guo, Ziping Zhang, Lili Yu,