Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5590631 | Infection, Genetics and Evolution | 2017 | 50 Pages |
Abstract
Bluetongue virus (BTV) is a member of the genus Orbivirus within the family Reoviridae and causes a non-contagious, insect-transmitted disease in domestic and wild ruminants, mainly in sheep and occasionally in cattle and some species of deer. Virus infection can trigger the changes of the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine which cellular miRNAs were differentially expressed in primary sheep testicular (ST) cells infected with BTV. A total of 25 known miRNAs and 240 novel miRNA candidates that were differentially expressed in BTV-infected and uninfected ST cells were identified, and 251 and 8428 predicted target genes were annotated, respectively. Nine differentially expressed miRNAs and their mRNA targets were validated by quantitative reverse transcription-polymerase chain reaction. Targets prediction and functional analysis of these regulated miRNAs revealed significant enrichment for several signaling pathways including MAPK, PI3K-Akt, endocytosis, Hippo, NF-kB, viral carcinogenesis, FoxO, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation on the roles of miRNAs in BTV replication and pathogenesis.
Keywords
PBSKEGG Orthology Based Annotation SystemBTVdsRNAsnoRNAsnRNAqRT-PCRSOAPCPEFBSMOIRNA interferenceSmall interfering RNAdouble-stranded RNARNAismall nuclear RNAsmall RNAsmall nucleolar RNAsiRNACytopathic effectRNA sequencingKEGG یا Kyoto Encyclopedia of Genes and Genomes microRNAsfetal bovine serumSRNAMEMModified Eagle's MediumPhosphate-buffered salineMicroRNAMiRNAGene ontologyplaque-forming unitsquantitative reverse transcription-polymerase chain reactionBluetongue viruspfumultiplicity of infectionKOBAS
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Authors
Junzheng Du, Shandian Gao, Zhancheng Tian, Shanshan Xing, Dexuan Huang, Guorui Zhang, Yadong Zheng, Guangyuan Liu, Jianxun Luo, Huiyun Chang, Hong Yin,