Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5590678 | Infection, Genetics and Evolution | 2017 | 23 Pages |
Abstract
Rolling-circle amplification-sequence-independent single primer amplifications (RCA-SISPA) and/or RCA-PCR-based approaches were applied to serial human plasma and animal (domestic cat) saliva samples. Complete SENV-H-related and PRA4 Anelloviridae genomes were characterized and analysed over time (~ 16 and 6.5 years for human and animal samples, respectively). Genomic sequences and deduced putative coding regions were compared. Comparable values, i.e. ~ 2 Ã 10â 4 subs/site/year, were obtained for estimated rates of non-synonymous substitutions. A “hot-spot” of mutations located on the SENV-H-related ORF1 was identified. These results are first data concerning Anelloviridae evolution in a human and an animal host based on the analysis of complete sequences.
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Authors
Sandra Bédarida, Bertrand Dussol, Michel Signoli, Philippe Biagini,